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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
Fecha : |
15/09/2014 |
Actualizado : |
26/11/2020 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
REYNO, R.; DONG-MAN, K.; MONTEROS, M.J.; BOUTON, J.H.; PARROTT, W.; BRUMMER, E.C. |
Afiliación : |
RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DONG-MAN, KHU; MARÍA J. MONTEROS; JOSEPH H. BOUTON; WAYNE PARROTT; E. CHARLES BRUMMER. |
Título : |
Evaluation of two transgenes for aluminum tolerance in alfalfa. |
Fecha de publicación : |
2013 |
Fuente / Imprenta : |
Crop Science, 2013, v. 53, no. 4, p. 1581-1588. DOI: https://doi.org/10.2135/cropsci2012.12.0676 |
DOI : |
10.2135/cropsci2012.12.0676 |
Idioma : |
Inglés |
Notas : |
History article: Received 3 Dec. 2012. Acknowledgments The authors would like to thank Donald Wood, Jonathan Markham, and Wesley Dean for their support with the greenhouse screening, Dr. Nathan Hancock for helping with the Southern blot analysis, Yanling Wei for laboratory assistance with gene expressions, and Maria Ortega for helping with the RT-PCR. We would also like to thank Forage Genetics Intl. for providing plant materials R2336 and 60T180-14. This study was funded by the Georgia Agricultural Experiment Stations and The Samuel Roberts Noble Foundation. |
Contenido : |
Alfalfa (Medicago sativa L.) production is dramatically reduced in acidic, Al-rich soil. Transgeni plants of several species overexpressing organic acid synthesis and/or organic acid transporter genes have shown enhanced tolerance to Al. The objective of this research was to evaluate the effect of the citrate synthase (CS) and the Daucus carota L. plasma membrane H+-transporting adenosine triphosphatase (H+ATPase) (DcPA1) transgenes on Al tolerance in alfalfa. Stem cuttings from a full-sib T2 population including four isogenic groups (plants with neither transgene, CS only, DcPA1 only,
and both transgenes) together with nontransformed check genotypes were evaluated for Al and acid soil tolerance in a greenhouse assay in limed and unlimed soil. Aluminum and acid soil tolerance was assessed by measuring the ratios of root and shoot dry weight in unlimed soil compared to limed soil. The three transgenic populations, CS, DcPA1, and CS plus DcPA1, all showed higher Al and acid oil tolerance and lower levels of Al in shoot tissue than the nontransgenic isogenic population or the nontransgenic parental genotypes. This suggests that an Al-exclusion mechanism could be driving Al and acid soil tolerance in this study. We observed no advantage of combining both transgenes in the
same genetic background. These transgenes offer an efficient method to achieve enhanced Al and acid soil tolerant alfalfa cultivars, but more information is needed on their stability across generations and genetic backgrounds and their performance under field conditions. MenosAlfalfa (Medicago sativa L.) production is dramatically reduced in acidic, Al-rich soil. Transgeni plants of several species overexpressing organic acid synthesis and/or organic acid transporter genes have shown enhanced tolerance to Al. The objective of this research was to evaluate the effect of the citrate synthase (CS) and the Daucus carota L. plasma membrane H+-transporting adenosine triphosphatase (H+ATPase) (DcPA1) transgenes on Al tolerance in alfalfa. Stem cuttings from a full-sib T2 population including four isogenic groups (plants with neither transgene, CS only, DcPA1 only,
and both transgenes) together with nontransformed check genotypes were evaluated for Al and acid soil tolerance in a greenhouse assay in limed and unlimed soil. Aluminum and acid soil tolerance was assessed by measuring the ratios of root and shoot dry weight in unlimed soil compared to limed soil. The three transgenic populations, CS, DcPA1, and CS plus DcPA1, all showed higher Al and acid oil tolerance and lower levels of Al in shoot tissue than the nontransgenic isogenic population or the nontransgenic parental genotypes. This suggests that an Al-exclusion mechanism could be driving Al and acid soil tolerance in this study. We observed no advantage of combining both transgenes in the
same genetic background. These transgenes offer an efficient method to achieve enhanced Al and acid soil tolerant alfalfa cultivars, but more information is needed on their stability across generations and ge... Presentar Todo |
Palabras claves : |
LUCERNE. |
Thesagro : |
ALFALFA. |
Asunto categoría : |
A50 Investigación agraria |
Marc : |
LEADER 02768naa a2200229 a 4500 001 1050225 005 2020-11-26 008 2013 bl uuuu u00u1 u #d 024 7 $a10.2135/cropsci2012.12.0676$2DOI 100 1 $aREYNO, R. 245 $aEvaluation of two transgenes for aluminum tolerance in alfalfa. 260 $c2013 500 $aHistory article: Received 3 Dec. 2012. Acknowledgments The authors would like to thank Donald Wood, Jonathan Markham, and Wesley Dean for their support with the greenhouse screening, Dr. Nathan Hancock for helping with the Southern blot analysis, Yanling Wei for laboratory assistance with gene expressions, and Maria Ortega for helping with the RT-PCR. We would also like to thank Forage Genetics Intl. for providing plant materials R2336 and 60T180-14. This study was funded by the Georgia Agricultural Experiment Stations and The Samuel Roberts Noble Foundation. 520 $aAlfalfa (Medicago sativa L.) production is dramatically reduced in acidic, Al-rich soil. Transgeni plants of several species overexpressing organic acid synthesis and/or organic acid transporter genes have shown enhanced tolerance to Al. The objective of this research was to evaluate the effect of the citrate synthase (CS) and the Daucus carota L. plasma membrane H+-transporting adenosine triphosphatase (H+ATPase) (DcPA1) transgenes on Al tolerance in alfalfa. Stem cuttings from a full-sib T2 population including four isogenic groups (plants with neither transgene, CS only, DcPA1 only, and both transgenes) together with nontransformed check genotypes were evaluated for Al and acid soil tolerance in a greenhouse assay in limed and unlimed soil. Aluminum and acid soil tolerance was assessed by measuring the ratios of root and shoot dry weight in unlimed soil compared to limed soil. The three transgenic populations, CS, DcPA1, and CS plus DcPA1, all showed higher Al and acid oil tolerance and lower levels of Al in shoot tissue than the nontransgenic isogenic population or the nontransgenic parental genotypes. This suggests that an Al-exclusion mechanism could be driving Al and acid soil tolerance in this study. We observed no advantage of combining both transgenes in the same genetic background. These transgenes offer an efficient method to achieve enhanced Al and acid soil tolerant alfalfa cultivars, but more information is needed on their stability across generations and genetic backgrounds and their performance under field conditions. 650 $aALFALFA 653 $aLUCERNE 700 1 $aDONG-MAN, K. 700 1 $aMONTEROS, M.J. 700 1 $aBOUTON, J.H. 700 1 $aPARROTT, W. 700 1 $aBRUMMER, E.C. 773 $tCrop Science, 2013$gv. 53, no. 4, p. 1581-1588. DOI: https://doi.org/10.2135/cropsci2012.12.0676
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4. | | FERRARI, V.; GIL, G.; HEINZEN, H.; ZOPPOLO, R.; IBÁÑEZ, F. Influence of Cultivar on Nutritional Composition and Nutraceutical Potential of Pecan Growing in Uruguay. Frontiers in Nutrition, 2022, volume 9, e868054. OPEN ACESS. doi: https://doi.org/10.3389/fnut.2022.868054 Article history: Received 02 February 2022; Accepted 30 May 2022; Published: 22 June 2022. -- Correspondence: Facundo Ibáñez, fibanez@inia.org.uy -- Edited by: Elena Ibañez, Institute of Food Science Research (CSIC), Spain. Reviewed by:...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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7. | | BESIL, N.; CESIO, V.; BOLOGNA, F.; LUQUE, E.; PINTOS, P.; RIVAS, C.; HEINZEN, H. Disipación de fungicidas e insecticidas utilizados en campo en el cultivo de mandarinas, naranjas y limones. In: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción Citrícola. Resultados de investigación en Citricultura: Genética, Sanidad, Productividad. Salto (Uruguay): INIA, 2015. p. 45-49 (Serie Actividades de Difusión; 752).Biblioteca(s): INIA Las Brujas. |
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Funding sources: Financial support from Instituto Nacional de Investigación Agropecuaria Uruguay,...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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10. | | BESIL, N.; PÉREZ-PARADA, A.; CESIO, V.; VARELA, P.; RIVAS, F.; HEINZEN, H. Degradation of imazalil, orthophenylphenol and pyrimethanil in Clementine mandarins under conventional postharvest industrial conditions at 4 °C. Food Chemistry, 2016, v.194, p. 1132-1137. Article history: Received 16 April 2015 / Received in revised form 25 August 2015 / Accepted 25 August 2015 / Available online 29 August 2015.
Chemical compounds studied in this article: Imazalil (PubChem CID: 37175) / Orthophenylphenol...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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12. | | PREVE, F.; ABELLA, I.; PEREIRA, D.; HEINZEN, H.; GONZÁLEZ, G.; PÉREZ, A. Lanas naturales y con bajo contenido de pesticidas. Agrociencia, 2009, v. 13, no. 3, p. 86Biblioteca(s): INIA Tacuarembó. |
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13. | | GÁMBARO, A.; ROASCIO, A.; HODOS, N.; MIGUES, I.; LADO, J.; HEINZEN, H.; RIVAS, F. The impact of sensory attributes of mandarins on consumer perception and preferences. Journal of Agriculture and Food Research, December 2021, volume 6, article 100196. OPEN ACCESS. doi: https://doi.org/10.1016/j.jafr.2021.100196 Article history: Received 20 November 2020, Revised 28 June 2021, Accepted 14 August 2021, Available online 19 August 2021, Version of Record 2 September 2021.
Corresponding author: Gámbaro, A.; General Flores, Uruguay;...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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14. | | MIGUES, I.; CESIO, V.; GÁMBARO, A.; MOYNA, G.; RODRÍGUEZ, C.; LADO, J.; RIVAS, F.; HEINZEN, H. NMR metabolomics as a prediction tool for consumers acceptance of mandarins. (SI-O-7) [oral presentation]. Session I: Breeding, Genomics and Genetics. In: RIivas, F. (Ed.). IV International Symposium on Citrus Biotechnology. Book of Abstracts. Montevideo (UY): INIA. p. 17. (INIA Serie Técnica; 244).Tipo: Presentaciones Orales |
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15. | | NIELL, S.; JESÚS, F.; PÉREZ, C.; MENDOZA, Y.; DIAZ, R.; FRANCO, J.; CESIO, V.; HEINZEN, H. QuEChERS adaptability for the analysis of pesticide residues in beehive products seeking the development of an agroecosystem sustainability monitor. Journal of Agricultural and Food Chemistry, 13 May 2015, Volume 63, Issue 18, Pages 4484-4492. Article history: Publication Date (Web): 16 Apr 2015Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : A - 1 |
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17. | | MIGUES, I.; RIVAS, F.; MOYNA, G.; KELLY, S. D.; HEINZEN, H. Predicting mandarin fruit acceptability: from high-field to benchtop NMR spectroscopy. Foods, 2022, volume 11, issue 16, article 2384. OPEN ACCESS. doi: https://doi.org/10.3390/foods11162384 Article history: Received 24 June 2022; Revised 29 July 2022; Accepted 1 August 2022; Published 9 August 2022.
Corresponding author: Heinzen, H.; Laboratorio de Farmacognosia y Productos Naturales, Departamento de Química Orgánica,...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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18. | | REZENDE, S.; BANCHERO, S.; MIGUES, I.; CESIO, M.V.; RIVAS, F.; HEINZEN, H.; BESIL, M.N. Variability levels of selected amino acids among mandarins produced in Uruguay. Ecletica Quimica, 2021, Volume 46, Issue 4, Pages 47-59. Gold Open Access. doi: https://doi.org/10.26850/1678-4618eqj.v46.4.2021.p47-59 Article history: Received: July 27, 2020; Accepted: July 16, 2021; Published: October 01, 2021.
Section Editor: Assis Vicente Benedetti.
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Biblioteca(s): INIA Las Brujas. |
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